Makedonka Mitreva, PhD
Associate Professor of Medicine
- BS: Ss. Cyril and Methodius University, Skopje, Macedonia (1990)
- MS: Ss. Cyril and Methodius University, Skopje, Macedonia (1994)
- PhD: Wageningen University and Research Centre, The Netherlands (2001)
- Postgraduate Reserach Associate: Dept of Genetics, Washington University School of Medicine, St. Louis Mentors: Dr. Robert Waterston and Dr. Richard Wilson (2004)
There are two main threads to my current research.
We study neglected tropical diseases (NTDs). There are 17 parasitic and bacterial infections that cause blindness, anemia, malnutrition, growth and development stunting in children, and severe morbidity and mortality during pregnancy in women. These infections can also increase the risk of co-infection with HIV/AIDS, tuberculosis and malaria. Their impact on health and socioeconomic status (mainly in the developing world) result in promoting and maintaining poverty. As the United Nations secretary general recently stated, “Poverty reduction and the elimination of NTDs go hand-in-hand.”
Our work translates basic science advances in genomics into practical knowledge that contributes to control or elimination of helminth NTDs (including hookworm, ascaris and whipworm, and flukes, including major biological carcinogens). My lab takes advantage of next-generation genomic and computational approaches to study:
i) taxonomically restricted and differentially represented pathways in parasites and their potential as targets for drug development,
ii) helminth genetic variations in natural infections,
iii) host response to parasite infections,
iv) identification of candidate antigens for vaccine or serodiagnostis,
v) molecular characterization of understudied parasite of global importance.
Through comparative pan-omics systems biology analysis we have provided fundamental molecular information for these devastating parasites of major medical and global importance accelerating both basic and translational research.
The second area of my research is focused on the human microbiome. My current efforts are to use targeted and shotgun sequencing of metagenomic communities of microbes to understand their biology. To study metagenomic communities through shotgun sequencing, advances in computational approaches and tools are needed, therefore we develop new and improve existing computational tools that will allow precise identification and comparison of taxonomic structure and functional capability of microbial communities in a robust fashion. We use those tools to identify genes, pathways and organisms within communities and use this information to determine what is common and variant among healthy and disease states, leading to identification of members or functions that are associated with different physiologic disease states.
Tyagi R, Maddirala AR, Elfawal M, Fischer C, Bulman C, Rosa BA, Gao X, Chugani R, Zhou M, Helander J, Brindley P, Tseng CC, Greig IR, Sakanari J, Wildman SA, Aroian R, Janetka JW, Mitreva M. Small molecule inhibitors of metabolic enzymes repurposed as a new class of anthelmintics. ACS Infect Dis. 2018 May 2. doi:10.1021/acsinfecdis.8b00090. [Epub ahead of print] PubMed PMID: 29718656. https://www.ncbi.nlm.nih.gov/pubmed/29718656
Rosa BA, Supali T, Gankpala L, Djuardi Y, Sartono E, Zhou Y, Fischer K, MartinJ, Tyagi R, Bolay FK, Fischer PU, Yazdanbakhsh M, Mitreva M. Differential human gut microbiome assemblages during soil-transmitted helminth infections in Indonesia and Liberia. Microbiome. 2018 Feb 28;6(1):33.. PubMed PMID: 29486796. https://www.ncbi.nlm.nih.gov/pubmed/29486796
McNulty SN, Tort JF, Rinaldi G, Fischer K, Rosa BA, Smircich P, Fontenla S, Choi YJ, Tyagi R, Hallsworth-Pepin K, Mann VH, Kammili L, Latham PS, Dell’Oca N, Dominguez F, Carmona C, Fischer PU4, Brindley PJ, Mitreva M.Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.PLoS Genet. 2017 Jan 6;13(1):e1006537. doi: 10.1371/journal.pgen.1006537. eCollection 2017 Jan.
Choi YJ, Tyagi R, McNulty SN, Rosa BA, Ozersky P, Martin J, Hallsworth-Pepin K, Unnasch TR, Norice CT, Nutman TB, Weil GJ, Fischer PU, Mitreva M.Genomic diversity in Onchocerca volvulus and its Wolbachia endosymbiont. Nat Microbiol. 2016 Nov 21;2:16207. doi: 10.1038/nmicrobiol.2016.207.
Gao X, Tyagi R, Magrini V, Ly A, Jasmer DP, Mitreva M.Compartmentalization of functions and predicted miRNA regulation among contiguous regions of the nematode intestine.RNA Biol. 2017 Oct 3;14(10):1335-1352. doi: 10.1080/15476286.2016.1166333. Epub 2016 Mar 22.